Category Archives: English

Searching for the best agarose candidate from genus Gracilaria, Eucheuma, Gelidium and local brands

Abstract

Objective

To explore the potential of local agar of genus GracilariaEucheumaGelidium and local brands as an alternative for imported agarose for DNA electrophoresis, and to examine their ability related to separation and migration of DNA fragments in DNA electrophoresis.

Methods

Their performance at various concentrations were compared via an experimental study with a specific brand of imported commercial agarose used in molecular biology research. The measured variables were separation and migration during electrophoresis of a DNA fragment.

Results

The local agar genus Gracilaria gigasGelidium, brand “B” and brand “S” could separate DNA fragments at a concentration between 1% and 2%, with an optimum concentration of 2% w/v, as good as a specific brand of imported commercial agarose.

Conclusions

Their performance were very close to that of commercial agarose and can still be improved by further agar purification as well as by pH and sulfur control.

Keywords

Agarose
Eucheuma
Gracilaria
Gelidium
Local brands
Indonesia agar

Suami SIAGA: male engagement in maternal health in Indonesia

Health Policy Plan czx073.
Published:
28 June 2017
Article history

Abstract

Suami SIAGA, which translates literally as the ‘alert husband’, is a national campaign that was created in early 2000 to promote male participation in maternal and child health program in Indonesia. The purpose of this study was to identify the proportion of men who took part in Suami SIAGA and the factors associated with their participation using the 2012 Indonesia Demographic and Health Survey (IDHS). This study also examined the relationship between Suami SIAGA and the following results related to the national campaign: the presence of husbands at antenatal care (ANC) units and the place of delivery at health facilities. Data on the characteristics of husbands and wives, as well as other related factors, the perceived elements of Suami SIAGA, and the national campaign outcomes were obtained from a total of 1256 eligible male subjects, drawn from the matched couples’ data set. The data was analysed using bivariate and multiple logistic regression to test the associations. This study found that 86% of the respondents were categorised as SIAGA husbands. After controlling all the variables, age and education of wife factors were significantly associated with Suami SIAGA, especially in the group of women aged 41–49 years old (OR = 2.4, 95% CI: 1.1–5.5) and women with a secondary level of education (OR = 1.8, 95% CI: 1.2–2.7) and higher (OR = 2.8, 95% CI: 1.4–5.6). SIAGA husbands were more likely to attend their wives’ ANC (OR = 2.3; 95% CI: 1.4–3.7). This study provides evidence on the benefit of husband involvement in maternal health, especially to improve ANC attendance. Empowering women themselves should also be addressed in leveraging the impact of Suami SIAGA.

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